Aliphatic nitrilase

aliphatic nitrilase
Identifiers
EC no.	3.5.5.7
CAS no.	9024-90-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, an aliphatic nitrilase also known as aliphatic nitrile aminohydrolase (EC 3.5.5.7) is an enzyme that catalyzes the hydrolysis of nitriles to carboxylic acids:

R-CN + 2 H₂O ${\displaystyle \rightleftharpoons }$ R-COOH + NH₃

Thus, the two substrates of this enzyme are an aliphatic nitrile (R-CN) and H₂O, whereas its two products are a carboxylic acid (R-COOH) and NH₃.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in nitriles. This enzyme participates in styrene degradation.

Kobayashi M, Yanaka N, Nagasawa T, Yamada H (1992). "Primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from Rhodococcus rhodochrous K22 and expression of its gene and identification of its active site residue". Biochemistry. 31 (37): 9000–7. doi:10.1021/bi00152a042. PMID 1390687.

• Pace HC, Brenner C (2001). "The nitrilase superfamily: classification, structure and function". Genome Biol. 2 (1): REVIEWS0001. doi:10.1186/gb-2001-2-1-reviews0001. PMC 150437. PMID 11380987.

3.5.1: Linear amides /
Amidohydrolases3.5.2: Cyclic amides/
Amidohydrolases3.5.3: Linear amidines/
Ureohydrolases3.5.4: Cyclic amidines/
Aminohydrolases3.5.5: Nitriles/
Aminohydrolases3.5.99: Other