Pitrilizin
| Pitrilizin | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.24.55 | ||||||||
| CAS broj | 81611-78-1 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Pitrilizin (EC 3.4.24.55, Escherichia coli proteaza III, proteaza Pi, proteinaza Pi, PTR, Escherichia coli metaloproteinaza Pi) je enzim.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Preferentno razlaganje -Tyr16-Leu- i -Phe25-Tyr- veza oksidovanog lanca insulina B. Takođe deluje na druge supstrate sa masom manjom od 7 kDa, kao što su insulin i glukagon
Ovaj enzim je prisutan u bakteriji Escherichia coli.
Reference
- ↑ Finch, P.W., Wilson, R.E., Brown, K., Hickson, I.D. and Emmerson, P.T. (1986). „Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III”. Nucleic Acids Res. 14: 7695-7703. PMID 3534791.
- ↑ Affholter, J.A., Fried, V.A. and Roth, R.A. (1988). „Human insulin-degrading enzyme shares structural and functional homologies with E. coli protease III”. Science 242: 1415-1418. PMID 3059494.
- ↑ Becker, A.B. and Roth, R.A. (1992). „An unusual active site identified in a family of zinc metalloendopeptidases”. Proc. Natl. Acad. Sci. USA 89: 3835-3839. PMID 1570301.
- ↑ Ding, L., Becker, A.B., Suzuki, A. and Roth, R.A. (1992). „Comparison of the enzymatic and biochemical properties of human insulin-degrading enzyme and Escherichia coli protease III”. J. Biol. Chem. 267: 2414-2420. PMID 1733942.
- ↑ Anastasi, A., Knight, C.G. and Barrett, A.J. (1993). „Characterization of the bacterial metalloendopeptidase pitrilysin by use of a continuous fluorescence assay”. Biochem. J. 290: 601-607. PMID 7680857.
Literatura
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.
Spoljašnje veze
- MeSH Pitrilysin
- p
- r
- u
Aktivno mesto • Alosterna regulacija • Mesto vezivanja • Katalitički perfektan enzim • Koenzim • Kofaktor • Kooperativnost • EC broj • Enzimska kataliza • Inhibicija enzima • Enzimska kinetika • Lajnviver–Burk dijagram • Mihaelis–Mentenova kinetika • Spisak enzima
EC1 Oksidoreduktaze/spisak • EC2 Transferaze/spisak • EC3 Hidrolaze/spisak • EC4 Lijaze/spisak • EC5 Izomeraze/spisak • EC6 Ligaze/spisak
B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6
